ColorFlux is a fast, reliable and non-toxic solution for assessing bacterial efflux for your research on antibiotic resistance. This coloured fluorescent compound quickly accumulates within bacteria as a function of their efflux pump activity. Simply add ColorFlux to your bacteria and watch them change colour ! 

How does ColorFlux work?

ColorFlux staining was shown to reflect the activity of well-characterized efflux pumps from the major facilitator superfamily and ATP-binding cassette families in a variety of Gram+ and Gram- bacteria:
- NorA, MepA, MepB, PatA, PatB (Staphylococcus aureus & Streptococcus pneumoniae)
- BmrA (Bacillus subtilis)
- AcrAB (Haemophilus influenzae)
The list will be updated regularly according to the feedback of users. 

Technical information about ColorFlux
Tested and validated on : Staphylococcus aureus, Bacillus subtilis, Streptococcus pneumoniae, Haemophilus influenzae. 
Colour: Red
Form: liquid
Detection methods: visual & fluorescence. Also compatible with single-cell approaches (live imaging and flow cytometry) 
Peak excitation/emission wavelengths: Ex:530nm/Em:650nm
Storage: at 4°C protected from light for 8 months

1 kit contains 1mL of an aqueous solution with a concentration of 1 mg/mL for a final volume of 1L of screening medium

ColorFlux staining of MepA&B mutants in Staphylococcus aureus

ColorFlux staining of NorA mutants in Staphylococcus aureus

ColorFlux staining of PatA/PatB mutants in Streptococcus pneumoniae

ColorFlux staining of BmrA mutants in Bacillus Subtilis

Distinguishing efflux activity levels across H. influenzae strains

Bacillus subtilis bacteria (moderate activity) stained with ColorFlux



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