GlowMito provides a non-toxic solution to quickly visualize the entire mitochondrial network in live samples. 

GlowMito quickly penetrates cells and produces a bright & stable red labeling of mitochondria without inducing cell toxicity or altering mitochondrial functions. Simply add it to your culture medium, and image repeatedly for up to 3 days without washing! 

Soluble in water and compatible with 2-photon microscopy, GlowMito is the solution of choice for thick tissues and in vivo experiments. 

GlowMito is retained in the mitochondria of live samples following loss of membrane potential. 

Technical Information about GlowMito

Colour: Red/near-infrared
Detection method : Fluorescence, one & two-photon excitation
Peak excitation/emission wavelengths: Ex:542nm/Em:690nm
Two-photon absorption peak: 790nm 
Two-photon cross-section: 224 GM
Form : Liquid (resuspended in water)
Sub-cellular localization: mitochondria
For use with: epifluorescence & confocal microscopes, flow cytometers                                
Fixable: no
 

Kit contents of GlowMito:

1 vial contains 125 µL of an aqueous solution with a concentration of 1 mM for a final volume of 250 mL of imaging medium
Storage: Store at 4°C for up to 7 months. For long-term storage, aliquot and store at -20°C. 

Applications of GlowMito:

Experimental outputs: live imaging & flow cytometry

GlowMito produces a bright red labeling of the entire mitochondrial network, including those with reduced potential. It is primarily intended to visualize mitochondria based on their presence rather than assessing their electrochemical properties. GlowMito does not need to be washed out: mitochondrial fluorescence will remain stable for up to 3 days without causing toxicity and can therefore be integrated in your long-term experiments. 

GlowMito is perfectly suitable for studying mitochondrial morphology, movement (velocity, localization), tracking, dynamics (fusion/fission), biogenesis (replication & growth), autophagy, etc.  

GlowMito can be used in combination with other probes (GFP, potentiometric dyes, calcium signaling probes, etc).

We do not recommend its use for the measure of mitochondrial mass or volume density.

• Tested and validated on cell lines, tissues & unicellular organisms: 
  
  - Humans: HEK293, HeLa, MCF-7, MDA-MB-231, HMLE, UACC-62, U2-OS, Gli36, HAEC, SH-SY5Y, A-172, A549, patient-derived skeletal muscle cells, primary smooth muscle cells & iPSC-derived cardiomyocytes, hiPSC-derived heart tissues. 
  - Mice: primary cortical neurons, acute brain slices, isolated pressurized blood vessels
  - Monkey: COS-7
  - Parasitic protists: Trichomonas Vaginalis (hydrogenosome labeling)

GlowMito showed no internalization in yeast cells, Entamoeba histolytica, Giardia intestinalis parasites and seedlings. 


Results:

Smooth muscle cell mitochondria labeling in an intact pressurized cerebral artery using GlowMito

 

Axonal mitochondrial labeling in acute mice brain slices using GlowMito

GlowMito produces a stable & bright labeling of mitochondria for 4 hours

Internalization & stability of GlowMito compared to a commercial mitochondrial probe 

Mitochondrial labeling in Aspergillus Fumigatus

Mitochondrial labeling in iPS-derived cardiomyocytes

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