How does it work?

Actiflash is a tamoxifen-like caged-inducer called cyclofen. Upon UV-light illumination, optical-induced uncaging of Actiflash quickly releases proteins fused to the modified estrogen receptor ligand-binding domains ERT2. This allows them to translocate into the nucleus, activate transcription (using Gal4-UAS system) or induce recombination (using the Cre-lox one).

The protein expression can thus be simply controlled in time and space thanks to UV-light!




Applications
Actiflash is a great R&D tool to convert your inducible ERT model into a photo-inducible one. It is very helpful if you want to control transcription (using Gal4-UAS) or induce recombination (using Cre-lox) in space and/or time for in-vivo cell tracking experiments and more.

Actiflash has been successfully used with various cell lines as well as zebrafish embryos. Actiflash will work best on zebrafish embryos from 12 to 48hpf. 

Find below a plasmid list that can be used with Actiflash. All these plasmids are available from Addgene.

Technical Information
Lifetime: upon receipt, Actiflash is stable for years at 2-8°C. Once solubilized in a DMSO solution (typically at 10 mM), Actiflash can be stored at -20°C for several months.  Always wrap the vials containing the aliquoted Actiflash with aluminium foil to protect it from light

 Additional resources:

>  ?Product overview?
>  ?Safety Datasheet?
>  ?Certificate of Analysis?
 

Visit our  ?glossary ? for a definition of Tamoxifen.