DESCRIPTION: Porcine type III collagen purified from pepsin-solubilized skin by repeat salt precipitation, DEAEcellulose, and Na2PO4 precipitation.
APPLICATION: Ideal as an ELISA antigen for detecting antibodies specific to porcine type III collagen in sera from experimental animals and humans, such as collagen-induced arthritis (CIA) models and patients with rheumatoid arthritis or other diseases, as well as normal controls to study the diversity of anti-collagen antibodies.
QUANTITY: 1 ml, 0.5 mg/ml
FORM: 0.5 mg/ml solution in 0.05M acetic acid
SOURCE: Porcine skin
MOLECULAR WEIGHT: 300 kD (consists of three α1(III) chains)
PURITY: >90% by 6% SDS-PAGE with 6M urea under reducing conditions (contaminated with less than 10% porcine type I collagen)
USAGE: Dilute this product with cold Collagen Dilution Buffer, (Catalog # 9003 as provided with the ELISA grade collagen) to prevent the aggregation of collagen molecules. For plate coating, take aliquots and dilute with the Collagen Dilution Buffer just before use. Do not store diluted solutions. The optimum coating concentration for ELISA plates is generally 5 mg/ml, but may vary based on the experimental purpose or the type of plate such as coating cell culture plates.
STORAGE TEMPERATURE: -20°C in the dark. This product is stable in acidic conditions at 4°C for several weeks and at -20°C for one year as supplied, but will gradually form polymeric collagen and degrade under neutral conditions.
STABILITY: 1 year