DESCRIPTION: Bovine type V collagen purifi ed from pepsin-solubilized amnion by repeating salt precipitation. Amnion type V collagen consisits of two α1 chains and one α2 chain, [α1(V)]₂ and α2(V).
APPLICATION: Use as an immunizing antigen to generate antibodies, an antigen to detect anti-type V collagen antibodies in ELISA, or as a standard for gel analysis.
QUANTITY: 1 mg
FORM: Lyophilized powder
SOURCE: Bovine
MOLECULAR WEIGHT: Intact amnion type V collagen: approximately 540 Kd. By 6% gel analysis, amnion type V collagen is separated into two chains: α1(V) and α2(V) (1840 and 1258 amino acid residues) from the top of the gel.
PURITY: >90% by SDS gel analysis
SOLUBILITY: Type V collagen can be dissolved at 4 mg/ml in acidic solution such as 0.01-0.05M acetic acid, pH 3.0-3.3 or 0.15M citrate buffer, pH 3.6 by stirring at 4°C overnight. To neutralize the solution, add 10x neutral buffer containing 1.5M NaCl or dialyze the solution against a neutral buffer.
STORAGE TEMPERATURE: 4°C in the dark for lyophilized form and -20°C for solution. Collagen might be gradually degraded under neutral conditions.
STABILITY: 2 years in lyophilized form
REFERENCES: Christopher Niyibizi et al. Human Placenta Type V Collagens. Journal of Biological Chemistry 259, No.22, 14170-14174 (1984)
M.Z. Abedin et al. Type V Collagen: The Presence of Appeciable Amounts of α3(V) chain in Uterus. Biochemical and Biophysical Research Communications 102 (4), 1237-1245 (1981).