DESCRIPTION: ELISA kits to quantify mouse anti-house dust mite (HDM) antibodies
FORMAT: Precoated 96-well ELISA Plate with removeable strips
ASSAY TYPE: Indirect ELISA
ASSAY TIME: 4.5 hours
STANDARD RANGE:
3030 (IgG) : 100 - 1.6 ng/ml
3034 (IgG1) : 100 - 1.6 ng/ml
3035 (IgG2b) : 10 - 0.16 ng/ml
3036 (IgM) : 1000 - 16 ng/ml
3038 (IgG2a) : 50 - 0.8 ng/ml
3039 (IgG3) : 1000 - 16 ng/ml
3046 (IgA) : 500 - 8 ng/ml
NUMBER OF SAMPLES: Up to 40 (duplicate) samples/plate
SAMPLE TYPES: Serum & Plasma (pre-treatment acceptable)
RECOMMENDED SAMPLE DILUTIONS: 1:100 (at least)
CHROMOGEN: TMB (read at 450 nm)
STORAGE: -20°C for 12 months
VALIDATION DATA:
3030: Intra-Assay (5.5-9.6%)/Inter-Assay (2.9-7.0%)/Spiking Test (100-109%)
3034: Intra-Assay (1.7-5.4%)/Inter-Assay (3.6-12.6%)/Spiking Test (90-106%)
3035: Intra-Assay (5.8-10.1%)/Inter-Assay (3.1-9.8%)/Spiking Test (103-111%)
3036: Intra-Assay (3.1-8.3%)/Inter-Assay (0.9-4.8%)/Spiking Test (102-105%)
3038: Intra-Assay (2.7-6.3%)/Inter-Assay (7.4-11.5%)/Spiking Test (87-96%)
3039: Intra-Assay (1.5-7.2%)/Inter-Assay (1.5-9.3%)/Spiking Test (100-109%)
3046: Intra-Assay (2.4-7.7%)/Inter-Assay (7.8-10%)/Spiking Test (96-100%)
NOTES: If serum samples require a lower dilution than 1:100, please contact support@chondrex.com