DESCRIPTION: ELISA kit to quantify mouse anti-ovalbumin antibodies
FORMAT: 96-well ELISA Plate with removeable strips
ASSAY TYPE: Indirect ELISA
ASSAY TIME: 4.5 hours
STANDARD RANGE:
3011 (IgG) : 12.5 - 0.2 ng/ml
3013 (IgG1) : 12.5 - 0.2 ng/ml
3015 (IgG2a) : 50 - 0.8 ng/ml
3016 (IgG2b) : 50 - 0.8 ng/ml
3017 (IgM) : 100 - 1.6 ng/ml
3018 (IgA) : 12.5 - 0.2 ng/ml
3029 (IgG2c) : 50 - 0.8 ng/ml
NUMBER OF SAMPLES: Up to 40 (duplicate) samples/plate
SAMPLE TYPES: Serum & Plasma
RECOMMENDED SAMPLE DILUTIONS: 1:100 (at least)
CHROMOGEN: TMB (read at 450 nm)
STORAGE: -20°C for 12 months
VALIDATION DATA:
3011: Intra-Assay (2.6-9.5%)/Inter-Assay (0.4-3.7%)/Spiking Test (93-107%)
3013: Intra-Assay (4.7-9.1%)/Inter-Assay (3.6-9.5 %)/Spiking Test (96-114%)
3015: Intra-Assay (5.9-8.9%)/Inter-Assay (6.3-9.4%)/Spiking Test (98-104%)
3016: Intra-Assay (3.9-9.9%)/Inter-Assay (6.3-9.4%)/Spiking Test (94-106%)
3017: Intra-Assay (2.9-4.6%)/Inter-Assay (6.6-9.1%)/Spiking Test (93-116%)
3018: Intra-Assay (0.2-7.8%)/Inter-Assay (1.2-7.9%)/Spiking Test (93-98%)
3029: Intra-Assay (5.5-9.6%)/Inter-Assay (2.9-7%)/Spiking Test (100-109%)
NOTES: N/A