DESCRIPTION: Mouse anti-OVA IgE monoclonal antibody, clone E-G5, unconjugated
APPLICATION: Use for animal studies, ELISA, and Western Blotting
QUANTITY: 1 mg containing 1 mg mouse serum albumin as a stabilizer (no preservative)
FORM: Lyophilized powder
SOURCE: Mouse
IMMUNOGEN: Ovalbumin
CROSS-REACTIVITY: Reacts to native OVA
SUBTYPE: Mouse IgE
PURITY: Purified by ion-exchange chromatography
USAGE: In vivo studies: Reconstitute antibody with PBS containing 1 mg/ml MSA or with Chondrex Inc.’s IgE dilution buffer (Catalog # 3009). In vitro studies: Reconstitute antibody with a buffer containing 2% heterologous serum, such as normal goat serum, to prevent non-specific reactions.
STORAGE TEMPERATURE: -20°C
STABILITY: 1 year
REFERENCES: N. Mizutani, H. Goshima, T. Nabe, S. Yoshino, Complement C3a-induced IL-17 plays a critical role in an IgE-mediated late-phase asthmatic response and airway hyperresponsiveness via neutrophilic inflammation in mice. J Immunol. 188, 5694–5705 (2012).
N. Mizutani, H. Goshima, T. Nabe, S. Yoshino, Establishment and characterization of a murine model for allergic asthma using allergen-specific IgE monoclonal antibody to study pathological roles of IgE. Immunology Letters. 141, 235–245 (2012).
N. Mizutani, C. Sae-Wong, S. Kangsanant, T. Nabe, S. Yoshino, Thymic stromal lymphopoietin-induced interleukin-17A is involved in the development of IgE-mediated atopic dermatitis-like skin lesions in mice. Immunology. 146, 568–581 (2015).
Different Results of IgE Binding- and Crosslinking-Based Allergy Tests Caused by Allergen Immobilization, 1–5 (2016).
C. Sae-Wong, N. Mizutani, S. Kangsanant, S. Yoshino, Topical skin treatment with Fab fragments of an allergen-specific IgG1 monoclonal antibody suppresses allergen-induced atopic dermatitis-like skin lesions in mice. European Journal of Pharmacology, 1–7 (2016).