DESCRIPTION: ELISA kits to quantify total mouse IgG/IgM/IgA immunoglobulins.
FORMAT: 96-well ELISA plate with removeable strips
ASSAY TYPE: Sandwich ELISA
ASSAY TIME: 4.5 hours
STANDARD RANGE: 100 ng/ml to 1.6 ng/ml
NUMBER OF SAMPLES: Up to 40 (duplicate) samples/plate
SAMPLE TYPES: Serum & Plasma (pre-treatment acceptable)
RECOMMENDED SAMPLE DILUTIONS: Varies (Starting point 1:100 or greater)
CHROMOGEN: OPD (read at 490 nm)
STORAGE: -20°C for 12 months
VALIDATION DATA:
3019 (IgA): Intra-Assay (3.2-7.9%)/Inter-Assay (3.3-8.5%)/Spiking Test (102-104%)
3023 (IgG): Intra-Assay (4.0-10.1%)/Inter-Assay (4.2-5.7%)/Spiking Test (93-113%)
3025 (IgG1): Intra-Assay (1.6-8.0%)/Inter-Assay (2.8-7.1%)/Spiking Test (93-100%)
3026 (IgG2a): Intra-Assay (8.3-9.0%)/Inter-Assay (4.1-6.9%)/Spiking Test (84-102%)
3027 (IgG2b): Intra-Assay (4.1-7.5%)/Inter-Assay (5.2-9.1%)/Spiking Test (93-100%)
3028 (IgG3): Intra-Assay (0.9-9.7%)/Inter-Assay (0.8-3.6%)/Spiking Test (86-97%)
3024 (IgM): Intra-Assay (2.3-6.5%)/Inter-Assay (2.7-5.1%)/Spiking Test (94-112%)
NOTES: N/A