DESCRIPTION: Mouse type V collagen purified from pepsin-solubilized intestine by salt precipitation. Type V collagen consisits of two α1 chains and one α2 chain, [α1(V)]₂ , and α2(V).
APPLICATION: Use as an immunizing antigen to generate antibodies, an antigen to detect anti-type V collagen antibodies in ELISA, or as a standard for gel analysis.
QUANTITY: 0.1 mg
FORM: Lyophilized powder
SOURCE: Mouse intestine
MOLECULAR WEIGHT: Intact type V collagen: approximately 540 Kd. By 6% gel analysis, type V collagen is separated into two chains: α1(V) and α2(V) (1840 and 1258 amino acid residues) from the top of the gel.
PURITY: >90% by SDS gel analysis
SOLUBILITY: Type V collagen can be dissolved at 4 mg/ml in acidic solution such as 0.01-0.05M acetic acid, pH 3.0-3.3 or 0.15M citrate buffer, pH 3.6 by stirring at 4°C overnight. To neutralize the solution, add 10X neutral buffer containing 1.5M NaCl or dialyze the solution against a neutral buffer.
STORAGE TEMPERATURE: 4°C in the dark for lyophilized form and -20°C for solution. Collagen might be gradually degraded under neutral conditions.
STABILITY: 2 years in lyophilized form
REFERENCES: Sato, K et al. Simple and Rapid Chromatographic Purification of Type V Collagen from a Pepsin Digest of Porcine Intestinal Connective Tissue, an Unmanageable Starting Material for Conventional Column Chromatography. Journal of Chromatography 790:277-283 (2003).