Fluorochrome公司是專業(yè)生產(chǎn)熒光金及熒光金抗體的專業(yè)高科技公司。公司核心產(chǎn)品熒光金于1985年推向市場，被廣泛應(yīng)用于神經(jīng)元逆行示蹤研究。熒光金在紫外線激發(fā)下發(fā)金黃色光，非常靈敏，不僅能標(biāo)記胞漿，而且能很好地顯示樹突分枝，但是細(xì)胞核和核仁不染色；在胞體內(nèi)分解慢，甚至在注射后存活2月標(biāo)記強(qiáng)度仍無明顯變化；比較耐紫外線的照射，褪色比較慢；可以經(jīng)受許多組織學(xué)染色處理，因而可以和HRP、免疫組織化學(xué)等方法結(jié)合。Fluorochrome公司還提供熒光金抗體及紅色熒光金，擴(kuò)大了其應(yīng)用范圍。
Fluoro-GoldTM 熒光金的優(yōu)勢(shì)：
1) 極其敏感和可靠的逆行示蹤劑
2)不會(huì)被未損害的通道纖維占據(jù)
3)細(xì)胞外無熒光滲漏，不易擴(kuò)散
4)保質(zhì)期長,熒光存在時(shí)間廣從1天到1個(gè)月
5)顯色效果清楚、逼真
6)安全且易操作
7)可壓力注射或電子透入動(dòng)物體
8)可以經(jīng)受多種組織學(xué)染色處理，因而可與其它常規(guī)神經(jīng)學(xué)檢測技術(shù)免疫熒光、免疫組化、射線自顯跡法、HRP組織化學(xué)及其他熒光逆行示蹤劑結(jié)合使用。
產(chǎn)品應(yīng)用實(shí)例：
保存：干粉，4℃避光保存，保質(zhì)期可超過1年。液體，4℃避光保存，保質(zhì)期6個(gè)月以上。
稀釋：熒光金可用雙蒸水或0.9%生理鹽水或0.2M的中性磷酸鹽緩沖液溶解。
訂購信息：
| 品牌 |
產(chǎn)品名稱 |
規(guī)格 |
| Fluorochrome |
Fluoro-Gold |
20mg（現(xiàn)貨） |
| Fluorochrome |
Fluoro-Gold |
50mg |
| Fluorochrome |
Fluoro-Gold |
100mg |
| Fluorochrome |
Fluoro-Gold |
150mg |
| Fluorochrome |
Fluoro-Gold |
200mg |
| Fluorochrome |
Antibody to Fluoro-Gold |
0.1ml×1 |
| Fluorochrome |
Antibody to Fluoro-Gold |
0.1ml×2 |
| Fluorochrome |
Antibody to Fluoro-Gold |
0.1ml×3 |
| Fluorochrome |
Fluoro-Ruby（紅色熒光金） |
30mg |
應(yīng)用文獻(xiàn)：
- A.P.Tosolini,et al.Spatial characterization of the motor neuron columns supplying the rat forelimb.Neuroscience,Volume 200, 3 January 2012, Pages 19-30
- E.van Asselt,et al.Spinal neurons in the zebrafish labeled with Fluoro-gold and wheat-germ agglutinin.Neuroscience Volume 43, Issues 2–3, 1991, Pages 611-622
- Lisa A. Catapano，et al.Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold. Neural Stem Cells pp 353-359
- LNovikova,et al.Persistent neuronal labeling by retrograde fluorescent tracers: a comparison between Fast Blue, Fluoro-Gold and various dextran conjugates.Journal of Neuroscience Methods Volume 74, Issue 1, 6 June 1997, Pages 9-15
- LC Schmued,et al.Fluoro-Gold: a new fluorescent retrograde axonal tracer with numerous unique properties.Brain Research Volume 377, Issue 1, 2 July 1986, Pages 147-154
- Junjie Yang,et al.Flow cytometry for quantification of retrogradely labeled retinal ganglion cells by Fluoro-Gold.Journal Current Eye Research Volume 21, 2000 - Issue 6
- AnnaPuigdell??vol-Sánchez,et al.On the use of fast blue, fluoro-gold and diamidino yellow for retrograde tracing after peripheral nerve injury: uptake, fading, dye interactions, and toxicity.Journal of Neuroscience Methods Volume 115, Issue 2, 15 April 2002, Pages 115-127
- Estudo comparativo entre enxerto autógeno e enxerto muscular coberto com tubo de veia autógeno em nervos tibiais de ratos wistar, utilizando fluoro-gold® como marcador neuronal,Acta ortop. bras. vol.15 no.2 São Paulo 2007
- Arthur HNeufeld,et al.The inherent, age-dependent loss of retinal ganglion cells is related to the lifespan of the species.Neurobiology of Aging.Volume 24, Issue 1, January–February 2003, Pages 167-172
- JavierRuiz-Ederra,et al.Mouse model of sustained elevation in intraocular pressure produced by episcleral vein occlusion.Experimental Eye Research Volume 82, Issue 5, May 2006, Pages 879-884
